Synthesis and Antimicrobial Activity of 5-chloro-1-ethyl-2-methylimidazole-4-sulfonyl-8-Quinolinoxide

Abstract

A new 5-chloro-1-ethyl-2-methylimidazole-4-sulfonyl-8-quinolinoxide was synthesized starting from diethyloxalate via a four-reaction step. The new compound obtained in a simple and efficient procedure in the solvent and solvent-free with good yield and methanol served as a precipitating agent to isolate compound <strong>2</strong>. The purity of 5-chloro-1-ethyl-2-methylimidazole-4-sulfonyl-8-quinolinoxide was tested by thin-layer chromatography (TLC) and characterized by Fourier transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (NMR), Carbon-13 (C13) nuclear magnetic resonance (¹³C-NMR), and DEPT 135 to confirm the presence and nature of CH₂. The solvent-free reaction of compound <strong>1</strong> was better in relation to green chemistry and better yield. The presence of solvent responsible for a color change that differentiates between compound <strong>1</strong> and <strong>2</strong> as they have the same physical data, functional group, and the chemical shift studied. The newly synthesized compound was evaluated and screened <em>in vitro</em> against Gram-positive<em> (methicillin-susceptible Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, </em>and<em> Bacillus subtilis)</em>, Gram-negative (<em>Pseudomonas aeruginosa, Escherichia coli,</em> and <em>Klebsiella pneumoniae) </em>bacterial strains, and <em>Candida albicans</em> using the standard microbiological method. 5-Chloro-1-ethyl-2-methylimidazole-4-sulfonyl-8-quinolinoxide exhibited weak activities when compared with the standard drug ciprofloxacin and itraconazole used for many bacterial and fungal infections respectively.