Effect of silver-cobalt nanoparticle on functional tests and liver tissue changes : an in vitro study

Abstract

Silver-cobalt nanoparticles are used in medicine. In this study, the effects of silver-cobalt nanoparticles on functional tests and liver tissue changes in adult male rats were investigated. 28 adult male Wistar rats weighing 180-220 g were divided into 4 groups of 7. Control group, Experimental group1 received 25mg / kg of silver-cobalt nanoparticles (AgCo NPs) in which silver nanoparticle (Ag NPs) were synthesized during 75 seconds intraperitoneally for 14 days. Experimental group 2 received 100 mg / kg of AgCo NPs in which Ag NPs were synthesized during 75 seconds intraperitoneally for 14 days. Experimental group 3 received 25 mg/kg of AgCo NPs in which Ag NPs were synthesized during 300 seconds intraperitoneally for 14 days. At the end of the test period, blood samples were taken from the heart and the serum levels of the liver enzymes were measured as AST, ALT, ALP, albumin and total protein. In addition, tissue changes in the liver were studied after hematoxylin-eosin staining. The results were analyzed by ANOVA and Duncan test. The results showed that the serum levels of aspartate aminotransferase (AST), albumin and total protein in experimental groups did not change significantly compared to the control group. Mean serum alanine aminotransferase (ALT) and alkaline phosphatase levels (ALP) in experimental groups did not change significantly compared to the control group (P <0.05). In the experimental groups receiving AgCo NPs, liver tissue damage was not observed. AgCo NPs do not change the serum levels of liver enzymes and liver tissue.