Synthesis and Cell Seeding Assessment of Novel Biphasic Nano Powder in the CaO–MgO–SiO2 System for Bone Implant Application

Abstract

<strong>Objective(s):</strong> CaO–MgO–SiO2 system bioceramics possess good characteristics for hard tissue engineering applications. The aim of the study was to synthesize the nano powder by using a sol-gel method and evaluate of bioactivity in the cells culture. <br /><strong>Methods:</strong> To characterize of powder X-ray diffraction (XRD), transmission electron microscopy (TEM) and to evaluate the bioactivity sample cell seeding and methylthiazol tetrazolium (MTT) assay were performed. <br /><strong>Results:</strong> X-ray diffraction (XRD) analysis showed that the biphasic powder was obtained at 1300°C for 2 h by using a sol-gel method. Transmission electron microscopy (TEM) image showed that powder particle size was about 45 nm. Besides, cell culture results indicated that the percentage of viability values was increased by the extension of period. <br /><strong>Conclusions:</strong> found that the sample is cytocompatible and has cell proliferation potential in culture medium. The present study demonstrates that, the biphasic CaO–MgO–SiO2 system can be used to achieve novel bioactive materials for bone implant application.