The anesthetic effects of 2-phenoxyethanol on Munzur trout fingerlings (Salmo munzuricus Turan et al., 2017) at different temperatures

Abstract

In this study, it was analyzed the anesthetic influences of 2-Phenoxyethanol (with %99 purity, BASF, Germany) on Munzur trout (Salmo munzuricus., a native species) originated from Munzur stream. 300 fish in total with the average weight of 4.42±0.62 g and length of 8.10±0.60 cm were used. To determine the anesthetic effects of various concentrations of 2-fhenoxyethanol on Munzur trout fingerlings, the experiments were performed in two different temperatures (13 ºC and 18ºC) and five different concentrations (0.1, 0.2, 0.3, 0.4 and 0.5 ml L-1). 2-Phenoxyethanol was used in this study as an anesthetic agent. In the anesthetic processing we practiced in all concentrations, except for 0.1 ml L-1 of 2-Phenoxyethanol, loss of reflex (S5) was determined between 68 sec and 276 sec, and total behavioral recovery (R3) was determined between 58 sec and 208 sec. Induction times of anesthesia varied with anesthetic concentrations, decreasing with the increase of 2- Phenoxyethanol concentrations. On the other hand, recovery times increased with decreasing of anesthetic concentrations. The lowest effective dose was determined as 0.3 ml L-1 at the temperatures 13 ºC and 18ºC. In this study, first time optimum anesthetic doses of 2-PE were determined for Salmo munziricus which is endemic to Munzur stream and has an importance for aquaculture.