Feeding Artemia larvae with yeast heat shock proteins 82 (HSPs82) to enhance the resistance against abiotic stresses (hyperosmotic and high temperatures)

Abstract

Feeding farmed <em>Artemia</em> with yeast heat shock proteins is a novel way to protect them from stress conditions during the culture. In this study, the effect of feeding with stressed new identified <em>Saccharomyces cerevisiae strain YG3-1</em> yeasts (containing induced heat shock proteins) on the survival of <em>Artemia</em>in stress conditions, was evaluated. For this purpose, heat shock proteins 82 (Hsps 82) of mentioned yeasts were induced by applying the high thermal (30, 35 and 40 ˚C) and high salinity (60, 120, 180 and 240 g.l^-1) stresses. After that, two different species of <em>Artemia</em> (<em>Artemia urmiana </em>and <em>A. franciscana</em>) were fed with treated yeasts during the culture. Then, to investigate the effects of <em>S. cerevisiae strain YG3-1</em> Hsps82 on <em>Artemia</em> survival, after the end of feeding, adult individuals of both species were exposed to authorized high salinity (230 g L^-1 and 280 g L^-1) and authorized high temperature (35 ˚C and 37 ˚C) as permitted stress for 48 h (hours). Finally, this administration resulted in the resistance of both species against the high salinity and high temperature (<em>p</em><0.05). This result was confirmed by analysing total protein of <em>Artemia</em> using SDS-PAGE, and suggests that this administration can be used for enhancing the survival of <em>Artemia</em> in stress conditions.