The invitro effect of Methyl tert-butyl ether on liver, gill and kidney tissues of Rutillus caspicus

Abstract

This study was conducted to evaluate the tissue of liver, gills and kidney Rutillus caspicus exposed to concentrations of 50, 100, 150 mg of Methyl tert-butyl ether, for 7, 14, 21 days. Average water temperature of 19±1 °C, water oxygen of 7.6 ± 0.2 mg/l and zero salinity were measured. A total of 156 fishes were studied in this experiment. In the first, second and third week of the test, three fishes were taken randomly from each aquarium. To examine the tissue, liver, gill and kidney were isolated, and using standard histological techniques, related microscopic slides were prepared for evaluation.<br /> <br /> Tissue damage in the liver includes: blood congestion، congestion of sinusoid، melano macrophage aggregation ، hepatocyte hypertrophy , vacuolation,degeneration، and cellular necrosis.<br /> <br /> Gill tissue damage includes: : hyperplasia، degeneration ,، lifting، telangiectasis،in secondary lamellae,blood congestion in primary & secondary lamellae، S formation of lamellae، reduction in length of secondary lamellae، lamellar fusion and cellular necrosis.<br /> <br /> <br /> <br /> Tissue damage in the kidney includes: tubular shrinkage، blood congestion ، melano macrophage aggregation،glomerular shrinkage، cellular necrosis، tubular degeneration، reduction in interstsial cells and interstisial hematopoietic tissue degeneration.<br /> <br /> <br /> <br /> The amount of damage in high concentrations of pollutants was higher, while gill , liver and kidney in the control group was observed in the normal outline. The result of this study showed that MTBE can cause damage in vital tissues of Rutillus caspicus and even, eventually leads to death.