High frequency <i>in vitro</i> propagation of M × M60, a cherry rootstock: the effects of culture media and growth regulators

Abstract

Effects of various culture media and growth regulators on micropropagation of cherry rootstock, M × M60 were investigated in the present study. Stem cuttings of the current season growth were taken from mature stockplants and cultured on the establishment medium after disinfection. After four weeks, elongated shoots were cut and subcultured on the proliferation media including MS, DKW, QL and ME supplemented with 0, 0.5, 0.7, 1 mg L^-1 of BAP. As a result, shoot number, average shoot length node number and internode length (except leaf area) were significantly affected by the interaction of medium with BAP level. The largest number of shoots was obtained in DKW (10.3 shoots/explants), ME (9.1 shoots/explants) and QL (9.1 shoots/explants) containing 0.7 mg L^-1 BAP. The results showed that DKW with 0.7 mg L^-1 BAP had the highest productivity compared to other treatments. For rooting, <em>in vitro</em> proliferated and elongated shoots were planted on LS, MS and ½ MS medium containing 0, 0.5, 1 and 1.5 mg L^-1 of IBA. High rooting percentage, root length and root number were obtained on ½ MS supplemented with 1 mg L^-1 IBA. The <em>in</em> <em>vitro</em> plantlets were transferred into the soil and the survival rate <em>ex</em> <em>vitro</em> was 95%.