Morphological and Molecular Identification of Botrytis Cinerea Causal Agent of Gray Mold in Rose Greenhouses in Centeral Regions of Iran

Abstract

Botrytis cinerea is an important pathogen that causes diseases in ornamental crops. In present<br />research several greenhouses of roses located in central region of Iran were surveyed to<br />identify the Botrytis cinerea. A total of 80 isolates were collected from rose greenhouses in<br />central region of Iran. Morphological identification was based on characters such as<br />conidiophore and conidial length. According to the results conidiophores length was (587)<br />657-3123 (3896) μm, conidial dimension were in the range of (4)8–13(16)×(2)4–7(10) μm.<br />Based on these characteristics, all isolates belonged to morphospecies Botrytis cinerea.<br />Using specific primer pairs C729 and PCR molecular method, a DNA fragment of 700bp<br />was amplified to the gene isolates for 30 representative isolates identified by morphological<br />assessment indicating the presence of Botrytis cinerea rose samples.