Isolation and characterization of Phi class glutathione transferase partial gene from Iranian barley

Abstract

Glutathione transferases are multifunctional proteins involved in several diverse intracellular events such as primary and secondary metabolisms, signaling and stress metabolism. These enzymes have been subdivided into eight classes in plants. The Phi class, being plant specific, is the most represented. In the present study, based on the sequences available at GenBank, different primers were designed for amplifying the Phi class of glutathione transferase gene in the genome and transcriptome of Iranian barley, Karoun cultivar. After extraction of DNA and total RNA, Phi class was amplified and sequenced. Bioinformatics analysis predicted that the deduced protein sequence has two ß-sheets, eight α-helices and some intermediate loops in its secondary structure. Consequently, the sequences were submitted to NCBI GenBank with GS262333 and GW342614 accession numbers. Phylogenic relationships of the sequences were compared with existing sequences in GenBank.