Phytochemical analysis, phenolic content and antioxidant activity of methanolic extract of Caryopteris odorata D.Don.Robin

Abstract

<strong>Background & Aim:</strong><em>Caryopteris odorata</em> is an aromatic shrub of family verbenaceae growing in tropical to subtropical regions. Various traditional applications of this plant have been documented guiding us to investigate the pharmacological activities and to further investigate the chemical composition.<br /> <strong>Experimental: </strong>Methanolic extract of aerial parts of <em>Caryopteris odorata </em>was investigated for antioxidant activity and the major phyto-constituents and total phenolic content were screened. The antioxidant activity was determined by measuring the scavenging activity using DPPH radical scavenging, NO radical scavenging, so radical scavenging activity, Metal chelating and reducing power assay. The antimicrobial efficacy was determined using agar well diffusion method against gram positive as well as gram negative bacteria. Sensitivity in terms of zones of inhibition and phytochemical composition of the extracts were also determined. <em>In vitro </em>anti-inflammatory activity was evaluated using albumin denaturation method. Diclofenec Sodium was used as a standard drug for the study of anti-inflammatory activity.<br /> <strong>Results:</strong> The results revealed that, methanolic extracts possess broad spectrum antibacterial activity against three microorganisms. Phytochemical analysis revealed the presence of diterpenes carbohydrates, glycosides, sterols and triterpenoids, aliphatic acids , tocopherols and phenolics. The methenolic extract exhibit strong antioxidant activity (IC₅₀ values for DPPH assay 164.60 ±0.37 mg/ml, for metal chelating 280.30 ± 3.28 mg/ml, for reducing assay 80.38 ± 0.79 mg/ml, respectively). The extract also showed <em>in vitro </em>anti-inflammatory activity by inhibiting the heat induced albumin denaturation with IC₅₀ value as 497.22 ± 1.34 mg/ml, respectively. From the result, it is concluded that the <em>C.odorata</em> possess a rich amount of different class of compounds and further the phytochemicals present in the <em>C.odorata </em>extract may be responsible for the antimicrobial, antioxidant and anti-inflammatory activity.<br /> <strong>Recommended applications/industries:</strong> The results obtained from various activities suggest <em>Caryopteris odorata</em> application as natural alternative antioxidant, anti-inflammatory and antimicrobial reagent.