The effect of Satureja bachtiarica on IL-6 and TNF-α in rat treated with Thioacetamide

Abstract

<strong>Background & Aim: </strong>Acute liver damage, inflammation and oxidative stress are underlying tissue necrosis and development of ciroces. The aim of this study was to evaluate the effect of <em>Satureja bakhtiarica</em> extract on acute liver disease induced by the thioacetamide and TNF-α and IL-6 cytokines level. <br /> <strong>Experimental: </strong>In experimental study, 36 Wistar rats were selected and divided into 6 groups of 6. The amount of 0.03g thioacetamide dissolved in 1ml of distilled water and injected intraperitoneally to all mice except of control group twice a week and for a period of three weeks. Negative control group received only thioacetamide and the other group received 8mg/kg sibilin by gavage in addition to thioacetamide. After injection of thioacetamide, the experimental groups were treated with doses of 5, 10 and 20 mg/ml extracts of <em>S. bakhtiarica</em>for two weeks. Peripheral blood samples were taken from rat hearts. Then TNF-α and IL-6 cytokines levels were measured by Elisa method. Histopathological changes of liver also were examined. Data analysis was performed with SPSS ver. 20.<br /> <strong>Results: </strong>The results showed a significant difference in TNF-α and IL-6 cytokines between groups (p<0.001). Mean concentration of TNF-α and IL-6 in the groups treated with the doses of 5(P = 0.002, P <0.001), 10 (P= 0.010, P <0.001) and 20 mg/ ml (P <0.001, P <0.001) of <em>S. bakhtiaricaca</em> significantly decreased compared with group treated with thioacetamide. Histopathological results also supported dose dependent protective effect of <em>S. bakhtiaricaca</em>. <em>S. bakhtiaricaca</em>extract has anti-inflammatory properties that can reduce the toxicity of thiouzamide by reducing the levels of TNF and IL-6 cytokines as pro-inflammatory cytokines.<br /> <strong>Recommended applications/industries:</strong>Regarding that the use of herbal medicines to treat many diseases are on the rise, hydroalcholic extract of Satureja bachtiarica due to having antioxidant properties can be used to treat liver disease with drug therapy.