Evaluating the effects of galbanic acid on hydrogen peroxide-induced oxidative DNA damage in human lymphocytes

Abstract

Objective: <em>Ferula szowitsiana</em> has been widely used for medicinal purposes around the world. The anti-oxidant effect of <em>F.szowitsiana </em>had been proved. The current study aims to determine the protective effects of galbanic acid, a sesquiterpene coumarin from <em>F. szowitsiana</em>, against hydrogen peroxide (H₂O₂) - induced oxidative DNA damage in human lymphocytes. <br/>Materials and Methods: Human lymphocytes were incubated with H₂O₂ (0, 25, 50, 100, and 200 µM), galbanic acid (200 and 400 µM) and a combination of galbanic acid (200 and 400 µM) and H₂O₂ (25 µM) at 4 C for 30 minutes. Solvents of galbanic acid without H₂O₂ were used as negative controls. <br/>Results: The findings of this study demonstrated that H₂O₂ exposure leads to a significant concentration-dependent increase in DNA damage. Galbanic acid did not cause DNA damage compared with the control cells. Data showed that galbanic acid does not have a protective effect against H₂O₂-induced oxidative DNA damage in human lymphocytes. <br/>Conclusion: According to the results, it is concluded that the capability of <em>F. szowitsiana </em>in reducing reactive oxygen species and the anti-inflammatory property of its methanolic extract may be due to its other ingredients.