Anticonvulsant effect of Satureja hortensis L. aerial parts extracts in mice

Abstract

<br/><strong>Objective: </strong>Regarding the anticonvulsant effects of <em>Satureja hortensis</em> (<em>S. hortensis</em>) in Avicenna's book: canon of medicine; the present study was undertaken to evaluate the anti- eplileptic effects of <em>S. hortensis</em> aqueous and ethanolic aerial part extracts. Furthermore, the mechanisms of their anticonvulsant activities were also evaluated. <br/><strong>Materials and Methods: </strong>Seizure was induced by Pentylentetrazol (PTZ) and MES (maximal electroshock) models. Mice were randomly divided into 8 groups; negative control (normal saline, 10ml/Kg), positive control (diazepam, 2 mg/kg), <em>S. hortensis </em>aqueous and ethanolic extracts (200, 400 and 600 mg/kg). In PTZ test, latency to the first minimal clonic seizure (MCS), latency to the first generalized tonic–clonic seizures (GTCS), the total duration of seizures and protection against mortality were evaluated. In MES test, the stretching length of extremities and protection against mortality were recorded. <br/><strong>Results: </strong>Aqueous and ethanolic extracts (400 and 600 mg/kg) significantly increased MCS and GTCS latencies in PTZ model. Three doses of the extracts decreased the total duration of seizure. These extracts did not show any protective effects on seizure induced by MES model. In PTZ model, flumazenil, an antagonist of benzodiazepine (BZD) site in the GABA_A-BZD receptor complex and 7- nitroindazole (7- NI), a selective nNOS (neuronal nitric oxide synthase) inhibitor, reduced the prolongation of seizure latency. <br/><strong>Conclusion: </strong><em>S. hortensis</em> showed anticonvulsant activity in PTZ model and this effect may be mediated, at least partly, through interacting with nitric oxide and GABA_A-BZD receptor complex.