Removal of Malachite Green by Using Immobilized Glucose Oxidase Onto Silica Nanostructure-Coated Silver Metal-Foam

Abstract

Enzymes Immobilization onto different types of the substrate could be helpful in various applications<br />of biomedical devices and biosensors. Enzyme activity and stability could be affected by support and<br />method of immobilization. In this study, the silver metal foam was successfully synthesized by the<br />soft-shell method and then was coated with silica. Then, glucose oxidase (GOx) immobilized on noncoated,<br />and silica-coated silver metal foam and removal of malachite green was investigated. Fourier<br />Transform Infrared Spectroscopy (FT-IR) and scanning electron microscopy (SEM) results confirmed<br />that the enzyme was attached to support surface. The maximum immobilized enzyme activity was<br />about 118.980 U/grSupport at 40°C. The removal of malachite green showed the indirect relation<br />with dye concentration confirmed by decolorization assay. The high activity, thermal stability, and<br />reusability of immobilized glucose oxidase onto the silver metal foam in comparison to free enzyme<br />introduce the capability of the biological system for removal of malachite green in the industry.