Effect of plant growth regulators on Fittonia verschaffeltii regeneration at in vitro conditions

Abstract

We investigated <em>in vitro</em> regeneration ability of <em>Fittonia</em> <em>verschaffeltii</em> using different explants (leaf, node) and plant growth regulators ((2,4-D, indoleacetic acid, indolebutyric acid (IBA), 6-benzylaminopurine (BAP), thidiazuron)). Percentage of callus induction, number of shoots, shoot length, percentage of rooting and root length were measured. The leaf explant showed higher frequency of callus induction in comparison with the node explant, while node had higher frequency of shoot formation. There were significant differences among plant growth regulators in terms of percentage of callus induction, number of shoots, percentage of rooting and root length, however, the difference was dependent on the type of explant. The highest percentage of callus induction (86.12%) was obtained for the leaf explant at Murashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-D and 1 mg/L IBA. The highest number of shoots (5.9 per explant) and shoot length (7.95 cm) were observed for the node explant at MS medium supplemented with 2 mg/L BAP + 1 mg/L IBA and MS medium containing 1mg/L BAP + 1 mg/L IBA, respectively. The highest percentage of root formation (87%) and root length (10.1 cm) were obtained at MS medium supplemented with 0.2 mg/L IBA and MS medium with no hormones, respectively.