Serum profiles of calcium, phosphorus, magnesium, vitamin D and parathyroid hormone in Caspian horses during different seasons

Abstract

Minerals play an essential role in the normal vital process.Calcium, phosphorus and magnesium are the most abundant minerals. Vitamin D and parathyroid hormone play a key role in regulation of their homeostasis as well. The aim of this study was to evaluate calcium, phosphorus, magnesium, vitamin D and parathyroid hormone on 30 Caspian horses of Guilan Province (south of the Caspian Sea) in two different seasons. So that, 15 stallions and 15 mares were sampled at the age groups ≤ 3 years and > 3 years, respectively. Fasting blood samples were collected twice, in August and January 2015 from jugular vein of horses. Serum calcium, phosphorus and magnesium were measured by colorimetric methods, while 25(OH)D3 and parathyroid hormone were measured by ELISA. The calcium (11.50 vs. 14.25 mg dL-1), magnesium (2.13 vs. 3.72 mg dL-1) and vitamin D (1.66 vs. 2.48 ng mL-1) levels were lower in winter than in summer (P < 0.05). The Caspian horses had higher phosphorus (4.52 vs. 3.26 mg dL-1) in winter than in summer (P < 0.05). Effect of sex on the measured parameters was not significant. Effects of age on the calcium, magnesium, vitamin D and parathyroid hormone levels were not significant, but ≤ 3 year - old horses had higher phosphorus (4.63 vs. 3.15 mg dL-1) than > 3 year - old ones (P < 0.05). The vitamin D level of mares was higher (3.10 vs. 1.43 ng mL-1) in summer than in winter (P < 0.05). Effects of sex, season, age and their interactions on parathyroid hormone were not significant. In conclusion, calcium, phosphorus, magnesium and parathyroid hormone levels in Caspian horses were within their physiological range, but vitamin D was low without any signs of deficiency. The Caspian horses had lower calcium, magnesium and vitamin D levels in winter than in summer.