Inheritance of RAPD marker in female grass carp (Ctenopharyngodon idella), male bighead carp (Hypophthalmichthys nobilis) and their F1 hybrids

Abstract

Genetic variation and inheritance of randomly amplified polymorphic DNA (RAPD) markers in female grass carp (Ctenopharyngodon idella) and male bighead carp (Hypophthalmichthys nobilis) and their F1 offspring hybrid have been studied. For this purpose, genomic DNA was extracted from muscle tissue according to phenol-chloroform method, and six decamer primers were used for amplifying polymorphic DNA. Results from 77 produced bands showed that all RAPD bands pattern of parents were present in F1 offspring hybrid, which indicated the high influence and dominance of the mentioned markers. The low difference in polymorphisms in used markers between F1 offspring hybrid and parents shows that parents are heterozygous in some loci, which can cause low difference in hybrids of two distinct genera. Although our results revealed that RAPD markers had a suitable efficiency in distinguishing parents from F1 hybrids, genetic diversity of triploid and diploid F1 hybrid were not detected by introduced primers.