A Piezoelectric Immunosensor for Early Cervical Cancer Detection

Abstract

<b>Background:</b> A piezoelectric immunosensor for early cervical cancer detection was developed involving shortanalyis time and less invasive technique for p16INK4a, a protein that has been linked to cervical cancer. Materialsand <br/><b>Methods</b>: 5μL of 5.0 mg/mL p16INK4a antibody and then supernatant from different clinical samples fromWest China Second University Hospital (Sichuan, China) were dripped on the center of the AT-cut crystalthrough a micro-injector. Absorption of the p16INK4a by antibody caused a shift in the resonant frequency ofthe immunosensor, and the resonant frequency was correlated to the amount of the p16INK4a in the supernatant.<br/><b>Results</b>: The greater severity of lesion grading, the greater the expression level of p16INK4. <br/><b>Conclusion</b>: Degree ofcervical cancer lesion development could be determined by detected amount of p16INK4a in different clinicalsamples.