miR-205 in Situ Expression and Localization in Head and Neck Tumors - a Tissue Array Study

Abstract

<b>Background:</b> microRNAs are small non-coding RNA that control gene expression by mRNA degradation ortranslational inhibition. These molecules are known to play essential roles in many biological and physiologicalprocesses. miR-205 may be differentially expressed in head and neck cancers; however, there are conflictingdata and localization of expression has yet to be determined. Materials and <br/><b>Methods</b>: miR-205 expression wasinvestigated in 48 cases of inflammatory, benign and malignant tumor tissue array of the neck, oronasopharynx,larynx and salivary glands by Locked Nucleic Acid in situ hybridization (LNA-ISH) technology. <br/><b>Results</b>: miR-205expression was significantly differentially expressed across all of the inflammatory, benign and malignant tumortissues of the neck. A significant increase in miR-205 staining intensity (p<0.05) was observed from inflammationto benign and malignant tumors in head and neck tissue array, suggesting that miR-205 could be a biomarkerto differentiate between cancer and non-cancer tissues. <br/><b>Conclusions</b>: LNA-ISH revealed that miR-205 exhibitedsignificant differential cytoplasmic and nuclear staining among inflammation, benign and malignant tumors ofhead and neck. miR-205 was not only exclusively expressed in squamous epithelial malignancy. This study offersinformation and a basis for a comprehensive study of the role of miR-205 that may be useful as a biomarkerand/or therapeutic target in head and neck tumors.