Amentoflavone Acts as a Radioprotector for Irradiated v79 Cells by Regulating Reactive Oxygen Species (ROS), Cell Cycle and Mitochondrial Mass

Abstract

Radioprotective effects of amentoflavone were investigated by examining cell viability, apoptosis, cell cyclingconcentrations of intracellular ROS (reactive oxygen species), and relative mitochondrial mass by flow cytometryafter 60Co irradiation. Pretreatment with amentoflavone 24 hours prior to 8 Gy 60Co γ-ray irradiation significantlyinhibited apoptosis, promoted the G2 phase, decreased the concentration of ROS and mitochondrial mass. Theseresults collectively indicate that amentoflavone is an effective radioprotective agent.