Association of 8 Loci on Chromosome 8q24 with Prostate Carcinoma Risk in Northern Chinese Men

Abstract

Multiple genetic studies have confirmed association of 8q24 variants with susceptibility to prostate cancer(PCa). As PCa risk SNPs may also influence disease outcome, we studied here eight 8q24 risk alleles, andevaluated their role in PCa clinical covariates in northern Chinese men. Blood samples and clinical informationwere collected from ethnically Chinese men from Northern China with histologically-confirmed PCa (n=289)and from age-matched normal controls (n=288). Eight 8q24 SNPs were genotyped by polymerase chain reactionhigh-resolution melting analysis in 577 subjects. We examined the prevalence distribution of 8q24 risk alleles andanalyzed the associations between the risk allele and PCa and clinical covariates to infer their impact on aggressivePCa. Three of the eight SNPs were associated with PCa risk in northern Chinese men, including rs16901966 (OR1.31, 95% CI 1.01-1.70, p=0.042), rs1447295 (OR 1.47, 95% CI 1.09-1.98, p=0.011) and rs10090154 (OR 1.55, 95%CI 1.14-2.12, p=0.005). Haplotype analysis based association with the risk alleles revealed significant differencesbetween cases and controls (OR 1.43, 95%CI 0.99-2.06, p=0.049). The risk alleles rs16901966, rs1447295 andrs10090154 were associated with age at diagnosis and tumor stage as compared with controls, while rs16901966was associated with aggressive PCa (OR 1.43, 95% CI 1.01-2.03, p=0.042). The evidence for 8q24 SNPs with PCarisk in northern Chinese men showed rs16901966, rs1447295 and rs10090154 at 8q24 (region 1, region 2) to bestrongly associated with PCa and clinical covariates. The three SNPs at 8q24 could be PCa susceptible geneticmarkers in northern Chinese men.