Quantitative Real-Time RT-PCR of ITGA7, SVEP1, TNS1, LPHN3, SEMA3G, KLB and MMP13 mRNA Expression in Breast Cancer

Abstract

Breast cancer is the leading cause of cancer deaths among women worldwide, including Thailand. In thepresent study, the differential mRNA expression of SVEP1, LPHN3, KLB, ITGA7, SEMA3G, TNS1 and MMP13genes was examined in breast cancer using quantitative real-time reverse transcription polymerase chainreaction (QRT-PCR). Among these genes, increased LPHN3 and MMP13 mRNA expression levels correlatedwith axillary-node metastasis (P=0.02). Multiple logistic regression analysis revealed that LPHN3 and MMP13mRNA expression is significantly associated with axillary node status in breast cancer (P=0.04).