Metabolomics Investigation of Cutaneous T Cell Lymphoma Based on UHPLC-QTOF/MS

Abstract

<br/><b>Objectives</b>: The identification of cutaneous T cell lymphoma (CTCL) biomarkers may serve as a predictor of disease progression and treatment response. The aim of this study was to map potential biomarkers in CTCL plasma. Design and <br/><b>Methods</b>: Plasma metabolic perturbations between CTCL cases and healthy individuals were investigated using metabolomics and ultrahigh performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS). <br/><b>Results</b>: Principal component analysis (PCA) of the spectra showedclear metabolic changes between the two groups. Thirty six potential biomarkers associated with CTCL were found. <br/><b>Conclusions</b>: Based on PCA, several biomarkers were determined and further identified by LC/MS/MS analysis. All of these could be potential early markers of CTCL. In addition, we established that heparin as a nticoagulant has better pre-treatment results than EDTA with the UHPLC-QTOF/MS appraoch.