Synaptic Vesicle Protein 2 (SV2) Isoforms

Abstract

New molecular markers of cancer had emerged with novel applications in cancer prevention and therapeutics,including for breast cancer of unknown causes, which has a high impact on the health of women worldwide. Thepurpose of this research was to detemine protein and mRNA expression of synaptic vesicle 2 (SV2) isoforms A, Band C in breast cancer cell lines. Cultured cell lines MDA-MB-231, SKBR3, T47D were lysed and their proteinand mRNA expression analyzed by real-time PCR and western blot technique, respectively. SV2A, B proteinswere identified in non-tumor (MCF-10A) and tumor cell lines (MDA-MB-231 and T47D) while SV2C only wasfound in the T47D cell line. Furthermore, the genomic expression was consistent with protein expression for asuch cell line, but in MDA-MB-231 there was no SV2B genomic expression, and the SV2C mRNA and proteinwere not found in the non tumoral cell line. These findings suggest a possible cellular transdifferentiation toneural character in breast cancer, of possible relevance to cancer development, and point to possible use of SV2as molecular marker and a vehicle for cancer treatment with botulinum toxin.