Interferon-γ and Interleukin-10 Gene Polymorphisms are not Predictors of Chronic Hepatitis C (Genotype-4) Disease Progression

Abstract

Immunoregulatory cytokines have an influence on hepatitis C virus (HCV) infection outcome. This studyaimed to determine whether single nucleotide polymorphisms (SNP) in IFN- γ and IL-10 genes are associatedwith susceptibility and/or are markers of prognosis regarding chronic hepatitis C outcomes. IFN γ (+874T/A)and IL-10 (-1082G/A) genotypes were determined in 75 HCV genotype 4 patients with different disease severities(chronic hepatitis, n=25, liver cirrhosis and hepatocellular carcinoma (HCC) on top of liver cirrhosis, n=50)and 25 healthy participants using allele-specific polymerase chain reaction. No statistical differences in alleleor genotype distributions of IFN γ and IL-10 genes were detected between patients and controls or betweenpatientgroups. No significant difference in the frequency of IL-10 SNP at position -1082 or IFN-γ at position+874T/A was found between chronic HCV genotype 4 and with progression of disease severity in liver cirrhosisor HCC. In conclusion; interferon-γ and interleukin-10 gene polymorphisms are not predictors of diseaseprogression in patients with chronic hepatitis C (Genotype-4).