AZD1480 Can Inhibit the Biological Behavior of Ovarian Cancer SKOV3 Cells in vitro

Abstract

<br/><b>Objective</b>: To study the mechanism of effects of AZD1480 on the SKOV3 ovarian cancer cell line. <br/><b>Methods</b>:The MTT method was used to assess cellular proliferation, flow cytometry for cellular apoptosis, the scratch testto determine migration, transwell chamber assays to detect cellular invasion, plate clone experiments to detectthe clone forming ability and Western blotting to determine p-STAT3 protein levels. <br/><b>Results</b>: The proliferationrate, migration ability, invasiveness and the clone forming ability of SKOV3 cells were reduced after treatmentwith AZD1480, while apoptosis rate and chemotherapeutic susceptibility were increased. After treatment withAZD1480 plus cisplatin, the apoptosis rate increased significantly while the expression level of p-STAT3 proteinwas decreased. <br/><b>Conclusion</b>: AZD1480 can inhibit the proliferation, invasion, metastasis and clone formation ofSKOV3 cells, induce cellulsar apoptosis, increase the chemotherapeutic sensitivity and reduce the expressionlevel of p-STAT3 protein.