Effect of miR27a on Proliferation and Invasion in Colonic Cancer Cells

Abstract

The aim of this study was to detect the expression of miR196a, miR146a, miR27a and miR200a in patientswith colon cancer, and investigate the effect of miR27a expression on proliferation and invasion in colonic cancercells. RT-PCR was employed to detect the expression levels in colon cancers. Then, colon cancer cells werecultured and transfected with 100 nM of miR27a mimics (80 nmol/L) or 80 nM miR27a inhibitors (80 nmol/L) in24-well plates. Proliferation and invasion of colonic cancer cells were then determined by CCK-8 and Transwellassays, respectively. Our data showed miR27a to be high-expressed in patients with colon cancer. In addition,proliferation and invasion in the miR27a mimic group were significantly higher than in the control group andnegative group (P<0.05), while, proliferation and invasion in the miR27a inhibitor group were obviously lowered(P<0.05). In conclusion, high expression of miR27a may play an important role in enhancing proliferation andinvasion of colon cancer cells.