Lack of Association of a Common Polymorphism in the 3’ -UTR of Interleukin 8 with Non Small Cell Lung Cancer in Kashmir

Abstract

<b>Background:</b> Chronic inflammation is considered as an important factor in the pathogenesis of lung cancer. Thepresence of inflammatory cells and higher levels of pro-inflammatory cytokines in the tumor microenvironmentand their surrounding tissues is gaining much importance in research. Materials and <br/><b>Methods</b>: One hundredninety NSCLC cases and 200 age, smoking and sex matched controls were evaluated for association of IL-8 -251(rs4073) and IL-8 -845 (rs2227532) in our population. Restriction fragment length polymorphism (RFLP) wasused followed by direct sequencing for the detection of SNPs. <br/><b>Results</b>: The IL-8 -845 polymorphism was notfound in our population. No significant association was observed between the IL-8 -251 AT genotypes and IL-8-25 AA genotypes and NSCLC (p=0.05) in our population. The IL-8 -251 A allele was also non-significant (p=0.05)in NSCLC patients. <br/><b>Conclusions</b>: In conclusion, this report reveals lack of association between IL-8 - 251 A/Tpolymorphism and NSCLC in our Kashmir Valley population.