Gene Expression Profiling of Non–Hodgkin Lymphomas

Abstract

<b>Background:</b> Chromosomal translocations are genetic aberrations associated with specific non–Hodgkinlymphoma (NHL) subtypes. This study investigated the differential gene expression profile of Egyptian NHLcases based on a microarray approach. Materials and <br/><b>Methods</b>: The study included tissue samples from 40 NHLpatients and 20 normal lymph nodes used as controls. Total RNA was extracted and used for cDNA microarrayassays. The quantitative real time polymerase chain reaction was used to identify the aberrantly expressedgenes in cancer. <br/><b>Results</b>: Significant associations of 8 up-regulated and 4 down-regulated genes with NHLwere observed. Aberrant expression of a new group of genes not reported previously was apparent, includingdown-regulated NAG14 protein, 3 beta hydroxy-delta 5-c27 steroid oxi-reductase, oxi-glutarate dehydrogenase(lipo-amide), immunoglobulin lambda like polypeptide 3, protein kinase x linked, Hmt1, and caveolin 2 Tetraprotein. The up-regulated genes were Rb binding protein 5, DKFZP586J1624 protein, protein kinase inhibitorgamma, zinc finger protein 3, choline ethanolamine phospho-transferase CEPT1, protein phosphatase, andhistone deacetylase-3. <br/><b>Conclusions</b>: This study revealed that new differentially expressed genes that may bemarkers for NHL patients and individuals who are at high risk for cancer development.