MiR-675 Promotes the Growth of Hepatocellular Carcinoma Cells Through Cdc25A Pathway

Abstract

<span style="text-decoration: underline;"><span style="font-size: small;">Background</span></span><span style="font-size: small;">: MicroRNAs (miRNAs) have fundamental roles in tumorigenesis. MiR-675 is upregulated in hepatocellular carcinoma(HCC) cells. However, the roles of miR-675 in hepatocellular carcinogenesis are still not fully elucidated. In this study, we focus on investigating the effect and mechanism of miR-675 in proliferation of HCC cells. <span style="text-decoration: underline;">Materials and Methods</span>: The cell proliferation was measured by MTT assays after transfection with miR-675 inhibitor and miR-675 mimics in HCC cells. The expression level of miR-675 was detected by real-time quantitative reverse transcription polymerase chain reaction. Protein expression of Cdc25A was measured by western blotting analysis. <span style="text-decoration: underline;">Results</span>: In MTT assays, overexpression of miR-675 promoted the proliferation of HCC cells(</span><em><span style="font-family: Times,Times; font-size: small;"><span style="font-family: Times,Times; font-size: small;">P</span></span></em><span style="font-size: small;"><em><span style="font-family: Times,Times; font-size: small;"><span style="font-family: Times,Times; font-size: small;">P</span></span></em><span style="font-size: small;"><em><span style="font-family: Times,Times; font-size: small;"><span style="font-family: Times,Times; font-size: small;">P</span></span></em><span style="font-size: small;"><em><span style="font-family: Times,Times; font-size: small;"><span style="font-family: Times,Times; font-size: small;">P</span></span></em><span style="font-size: small;"><em><span style="font-family: Times,Times; font-size: small;"><span style="font-family: Times,Times; font-size: small;">p</span></span></em><span style="font-size: small;"><0.05) after treatment with miR-675 mimics. The expression level of Cdc25A was significantly decreased (</span><em><span style="font-family: Times,Times; font-size: small;"><span style="font-family: Times,Times; font-size: small;">p</span></span></em><span style="font-size: small;"><0.05) after treatment with miR-675 inhibitor. <span style="text-decoration: underline;">Conclusions</span>: Our results indicate that miR-675 promotes the proliferation in human hepatocellular carcinoma cells by associating with Cdc25A signaling pathway. </span></span></span></span></span>