Mitochondrial D-Loop Polymorphism and Microsatellite Instability in Prostate Cancer and Benign Hyperplasia Patients

Abstract

In this study mitochondrial D-Loop variations in Iranian prostate cancer and benign prostatic hyperplasia(BPH) patients were investigated. Tumour samples and corresponding non-cancerous prostate tissue from 40prostate cancer patients and 40 age-matched BPH patients were collected. The entire mtD-loop region (16024-576) was amplified using the PCR method and products were gel-purified and subjected to direct nucleotidesequencing. A total of 129 variations were found, the most frequent being 263AgG and 310TgC among bothBPH and prostate cancer patients. Variation of 309 CgT was significantly more frequent in prostate cancerpatients (P value<0.05). Four novel variations were observed on comparison with the MITOMAP database.Novel variations were np16154delT, np366GgA, np389GgA and 56insT. There was no correspondence betweenthe different variations and the age of subjects. Considering that D-loop variations were frequent in both BPHand prostate cancer patients in our study, the fact that both groups had high average age can be a possiblecontributing factor. D-loop polymorphisms and microsatellite instability can influence cell physiology and resultin a benign or malignant phenotype. Significantly higher frequency of 309 CgT variation in cancer patients isa notable finding and must be a focus of attention in future studies.