Lack of Association between Chlamydia trachomatis Infection and Cervical Cancer - Taq Man Realtime PCR Assay Findings

Abstract

<b>Background:</b> Cervical cancer is one of the most common cancers in developing countries and the secondmost common type of cancer in women globally. Several recent studies suggested a co factor role for Chlamydiatrachomatis in pathogenesis of cervical cancer. This study aimed to evaluate existence of C. trachomatis DNAin pathologic blocks of patients with cervical cancer. Materials and methods: Seventy-six formaldehyde fixedparaffin embedded tissue specimens from patients with histologically proven history of cervical cancer as wellas 150 blocks from healthy peoples were included in the present study. Thin slices were prepared from selectedblocks followed by deparaffinization and DNA extraction; the presence of C. trachomatis DNA was examinedby Taq Man real-time PCR. <br/><b>Results</b>: Our TaqMan real time PCR assay with cervical specimens of patients withcervical cancer showed that there was no C. trachomatis DNA. Also, we found three positive specimens among ourcontrol group. <br/><b>Conclusion</b>: It seems that based on results obtained from the specimens examined in the presentstudy, there is no association between the presence of C. trachomatis DNA in cervical specimens and cervicalcancer.