Application of Magnetic Polymer Particles Modified with β–Cyclodextrin for Adsorption of Bovine Serum Albumin

Abstract

Magnetic polymer particles which were modified by vinyl groups and subjected to<br />polymerization by vinyl β-Cyclodextrin derivative, has been used as adsorbent for sorption and<br />release of bovine serum albumin and the equilibrium and kinetics of the adsorption process were<br />studied. The absorbability and releasing of this protein through the new polymer has been<br />measured by ultraviolet-visible spectroscopy. Effects of various parameters such as pH, contact<br />time and capacity for adsorption of bovine serum albumin have been studied in vitro and the<br />optimum magnitudes were 4, 37.94 mg/g and 5 minutes respectively. The experimental results<br />show that the adsorption of Bovine serum albumin on magnetic particles was affected greatly by<br />the pH andthe adsorption equilibrium isotherm was fitted well by the Freundlich model. Bovine<br />serum albumin was released from magnetic particles in simulated intestinal fluids. It was<br />released up to about 7 h at high speed and then it released slowly up to 30 h.<br /><br />