Selective and Validated SpectrophotometricMethods for Determination of Acyclovir and Ganciclovirwith 2, 4 as Reagent

Abstract

New, simple and sensitive spectrophotometric methods for the determination of acyclovir and ganciclovirhave been developed. The method is based on the oxidation of 2, 4-dinitrophenyl hydrazine (2, 4 DNP) and coupling of the oxidized product with acyclovir and ganciclovirto give intensely coloredchromogen. Acyclovir and ganciclovirshowed maximum absorbance at 414 nm and 450 nm with linearity was observed in the concentration range of 20-60 µg mL-1 and 5-25 µg mL-1 respectively. The relative standard deviations of 0.016 for acyclovir and 0.014 ganciclovirwere obtained. The recoveries of acyclovir and ganciclovirtablets are in the range98.48, 99.28respectively. The proposed method is simple, rapid, precise and convenient for the assay of acyclovir and ganciclovirin commercial tablet preparations.