MicroRNA-215 as a Diagnostic Marker in Egyptian Patients with Hepatocellular Carcinoma

Abstract

Background: MicroRNAs are mentioned as a small non-coding RNAs groups and aberrant miRNA expression was<br />found in hepatocellular carcinoma (HCC) patients. Aim: To evaluate role of plasma MicroRNA-215 as a diagnostic<br />tool in HCC patients. Methods: A prospective study included 195 subjects: healthy controls (group I), cirrhotic patients<br />(group II), and patients with HCC (group III). Clinical examination, radiological and laboratory investigations which<br />included quantification of miR-215 by Real-time qPCR were done for all cases. Results: Spearman's rank correlation<br />revealed that in HCC group, there was a negative correlation between MiRNA-215 and serum AFP levels and focal size<br />lesion (cm) (rs = -0.72, - 0.94 respectively, p<0.001). Receiver operating characteristics analysis for discrimination<br />between cirrhosis and HCC groups regarding microRNA-215 displayed 78.3% sensitivity, 88.0% specificity at cutoff<br />value of ≤ 1.90. Area under the curve (AUC) was 0.87 (p< 0.001). As regards AFP, it had a sensitivity of 81.7%, a<br />specificity of 66.7 at cutoff value of ≥ 11.50 (ng/mL). Conclusions: Plasma level of miR-215 may be a promising<br />biomarker in HCC diagnosis. Moreover, if miR-215 combined with AFP, it can be used as a diagnostic biomarker, for<br />early detection of HCC.