Genetic Diversity between Bali Cattle (<i>Bos javanicus</i>) and It’s Hybrids Using Microsatellite Markers

Abstract

The aim of this study was to evaluate the Bali cattle genetic diversity and hybrid cattle using microsatellite markers. Blood samples (n=192) were collected from various cattle breed, i.e. Bali (n=96), Madura (n=48), and Peranakan Ongole (PO) Kebumen (n=48). The microsatellite locus used for assessing the genetic variation was INRA035, ILSTS006, ETH225, and HEL9, while the genetic profile was described using GenAlEX, Cervus, MEGA6, structure and R programs. As a result, 46 alleles were found at four microsatellite loci studied. The genetic diversity of Bali cattle from Bali island (H_o=0.337) and Nusa Penida island (H_o=0.375) were recorded lower than that of the hybrids, i.e. Madura (H_o=0.747) and PO Kebumen (H_o=0.567) cattle breeds. Specifically, we found 283 bp (locus ILSTS006), 194 bp (locus ETH225), 147 bp and 151 bp (locus HEL9) demonstrating the distinctive alleles for Bali cattle. Also, our experimental data showed that microsatellite markers used allowed us to produce an obvious differentiation of the cattle cluster between Bali cattle and the hybrids, which was meaningful for future cattle breeding program.