VEGF-C Antisense Oligoxydeonucleotide Suppression of Invasive Ability of the A-549 Lung Carcinoma Cell Line

Abstract

<br/><b>Objective</b>: Vascular endothelial growth factor C (VEGF-C), a member of the VEGF family, has been reported to promote angiogenesis and tumor cell growth. In this study, we analyzed inhibitory action of a VEGF-C antisense oligoxydeonucleotide (ASODN) on a lung carcinoma cell line A-549 and its invasive ability in vitro. <br/><b>Methods</b>: Liposomes, liposome-mediated sense oligoxydeonucleotide (SODN) and ASODN were transfected into A-549 cells, with a blank control group. The expression of VEGF-C was examined by western blotting and invasive ability of cells was detected at four levels. <br/><b>Results</b>: Lower expression of VEGF-C and lower invasive ability to recombinate basal membranes were apparent in the ASOND group (P<0.01). However, there were no significant differences among the other three groups (P>0.05). In the invasion assay, the number of transmembrane cells was significantly reduced in the ASODN group after 48 hours (58.6±9.2 P<0.01), but there was no variation amongcontrol, LIP and SOND groups (132.5±15.6, 129.7±16.1, 118.2±12.5, P>0.05). <br/><b>Conclusions</b>: VEGF-C ASODN can downregulate the expression of VEGF-C in cell lines and can obviously inhibit invasive ability in vitro.