Evaluation of Therapeutic Potential of Eugenol-A Natural Derivative of Syzygium aromaticum on Cervical Cancer

Abstract

Background: The intendment of this study is to determine the pursuance in – vitro anticancer activity and<br />cytotoxicity of Syzygium aromaticum against the human cervical cancer cell line (HeLa) compared to the normal cell<br />lines. Apoptogenic properties of DCM extract of Eugenol was determined in this entire study. Materials and Methods:<br />HeLa cell lines were cultured in DMEM medium and incubated with different concentration of DCM – Eugenol<br />extract. MTT assay brought out the way to determine the cell viability and quantification was done with the optical<br />absorbance at 570 nm and 620 nm as reference. Apoptotic cells were affirmed by dual staining using acridine orange<br />bromide. Besides, the morphology of the nucleus was also confirmed by dual staining. Eugenol inhibited 50% growth<br />(IC50) of HeLa cell lines at 200 mg/ml of extract concentration. Results: Inhibitory efficacy of eugenol isolated from<br />Syzyzgyium aromaticum showed the cell – viability in time and dose dependent manner with consistent morphological<br />changes. Flow cytometer determined the apoptosis confirming the cytotoxicity value for MTT at IC50 with 81.85% cell<br />viability. Dual staining firmly enacts the damaged cells due to AO indicating apoptosis confirmation by dual staining.<br />Morphological analysis also clearly states that nil apoptosis has been seen in control and similarly in eugenol treated<br />when compared to cancerous HeLa cell – line. Conclusion: Evaluation of cytotoxicity effect of eugenol isolated from<br />Syzygium aromaticum showed it can be unrivalled dormant source of prodigious changes in HeLa cell line indicating<br />(revealing) that chemotherapeutic agent.