Expression of MiR200a, miR93, Metastasis-related GeneRECK and MMP2/MMP9 in Human Cervical Carcinoma -Relationship with Prognosis

Abstract

Aim and <strong>Background:</strong> Cervical cancer remains the third most common cancer in women globally afterbreast and colorectal cancer. Well-characterized biomarkers are necessary for early diagnosis and to predictmetastatic progression and effective therapy. MiRNAs can regulate gene expression, cell growth, differentiationand apoptosis by targeting mRNAs for translational repression or degradation in tumor cells. The present studywas conducted to assess expression of miR93, miR200a, RECK, MMP2, MMP9 in invasive cervical carcinoma,and analyze their clinical significance. <br /><strong>Method</strong>: A total of 116 patients with invasive cervical carcinoma and 100patients undergoing hysterectomy for benign lesions were retrospectively examined. Quantitative real-time PCRwas performed to determine expression of miR93 and miR200a while RECK, MMP2, MMP9 and MVD wereassessed by immunohistochemical staining. <br /><strong>Results</strong>: Cervical carcinoma patients demonstrated up-regulationof miR-93, miR-200a, MMP2 and MMP9, with down-regulation of RECK as compared to benign lesion tissues.RECK was significantly inversely related to invasion and lymphatic metastasis. The 5-year survival rate forpatients with strong RECK expression was significantly higher than that with weakly expressing tumors.<br /><strong>Conclusion</strong>: MiR-93 and miR-200a are associated with metastasis and invasion of cervical carcinoma. Thustogether with RECK they are potential prognostic markers for cervical carcinoma. RECK cooperating withMMP2, MMP9 expression is a significant prognostic factor correlated with long-term survival for patients withinvasive cervical carcinoma.