Electrochemiluminesence Epidemiologic Detection of Toxoplasma gondii Infection in Pregnant Women With Direct and Indirect Diagnostic Techniques (ELISA Avidity Plus Biochemical Assay)

Abstract

Background and aims: Toxoplasmosis is a very common disease in the world. Two types of acquired<br />toxoplasmosis have been detected. In the chronic toxoplasmosis, the abnormality of tissue function<br />is little but, in acute toxoplasmosis, function of RES system becomes interrupted. The assessment of<br />toxoplasma antibody with tissue enzymes in this stage is very important. Furthermore, serum ferritin<br />in some conditions became high in acute phase of infectious disease.<br />Methods: This study was based on comparative abundance study. Materials consisted of 980 serum<br />and amniotic fluid samples collected from human blood with high level of IgG antibody against<br />Toxoplasma gondii in Rajaie center, Tehran, Iran. The standard test was ELISA assay to detect these<br />antibodies and the main test was measurement of liver transaminases (SGOT, SGPT) bilirubin and<br />ferritin to evaluate acute toxoplasmosis. Data analysis was done by SPSS version 18.0. This study was<br />done during March to June, 2017.<br />Results: Results showed that in some patients with high level of IgG AVIDITY antibody against T. gondii<br />the level of liver transaminases, serum bilirubin and ferritin became increased. For example in 120<br />patients with acute toxoplasmosis the mean serum levels of SGOT were 108 IU/L and in 80 patients<br />the mean serum bilirubin were about 5 mg/dL.<br />Conclusion: In acute congenital toxoplasmosis, the evaluation of IgG AVIDITY was first step and then<br />the measurement of biochemical factors such as serum transaminases, serum Bilirubin and serum<br />Ferritin were important.