Calotroposid A: a Glycosides Terpenoids from Calotropis gigantea Induces Apoptosis of Colon Cancer WiDr Cells through Cell Cycle Arrest G2/M and Caspase 8 Expression

Abstract

Objective: This study aims to isolate the active anticancer compound from ethyl acetate fraction extracted from<br />the roots of Calotropis gigantea and to determine the operating mechanism of the isolates towards WiDr colon cancer<br />cells. Methods: the isolation was conducted by using bioassay guided isolation approach method. The cytotoxic<br />potential was determined by using MTT method. The chemical structure was identified by using UPLCMS/MS and<br />NMR-1H spectroscopy. The cell cycle arrest and apoptosis induction were determined by flow cytometry method.<br />The expression of caspase-8 was determined by immunocytochemistry method. Results: The results showed that<br />the active compounds are obtained calotroposid A compound which is glycosides terpenoids. Calotroposide A<br />is capable of inhibiting the growth of WiDr colon cancer cells at IC50 17.23μg/ml. Cell apoptosis induction took place<br />and was indicated by cell apoptosis increase, S and G2/M accumulation and by caspase-8 expression. Conclusion:<br />Calotroposide A induces anticancer activity against WiDr colon cancer cells by means of apoptosis induction mechanism<br />through extrinsic pathway with increased expression of caspase-8.