Isolation and Detection of Virulence factors of Salmonella typhimurium and Salmonella enteritidis

Abstract

Plasmids play an important role in the virulence and antibiotic resistance of <em>Salmonella</em>. <em>Salmonella</em> carry plasmids having different size and number. Plasmid profile analysis of single isolates of <em>Salmonella</em> <em>typhimurium</em> and <em>Salmonella</em> <em>enteritidis</em> revealed the presence of 4- plasmid profiles. <em>Salmonella</em> serovars produce several Type III secretions including Sop-E and Sop –B. Soap E is detected by Dot-ELISA. Dot-ELISA is one of the solid phase immuneassay used to detect either antigens or antibody. Sop-B (<em>Salmonella </em>outer protein-B) is detected by multiplex PCR method using PCR primers <em>Sop P1</em> and <em>SOP- M13.</em> It is concluded that the technique used in this study is more sensitive and identification and determination of various molecular characteristics of two species enhances the knowledge towards the vaccine production.