Assessment of adaptive response of gamma radiation in the operating room personnel exposed to anesthetic gases by measuring the expression of Ku 80, Ligase1 and P53 genes

Abstract

<strong>Introduction</strong>: Staffs of operating room are continuously exposed to anesthetic gases and <span style="text-decoration: underline;"> </span> ionizing radiation. Adaptive response, as a defense mechanism, will occur when cells become exposed to a low dose of factors harming DNA that causes in the next exposures to higher doses of those factors, more resistance and sensibility can be observed. The aim of this study was to investigate adaptive response or synergistic effects of ionizing radiation in the operating room personnel exposed to anesthetic gases by evaluation of the relative gene expression changes of effective genes for DNA repair such as KU80, LIGASE1 and P53. <strong>Materials and Methods: </strong>In this study, 20 people of operating room personnel (12 men and 8 women) with an average age of (34/35±7/33), and 20 people from the personnel of other wards (12 men and 8 women) with an average age of (34/05±6/50) were selected as the control group. For every individual's 2cc heparin blood, RNA extraction and cDNA synthesis were performed. In order to study the adaptive response, a challenge dose of 2Gy gamma radiation was used. Expression level of the genes was studied by Real-Time PCR method and compared with that of the control group. The results were statistically analyzed using SPSS software. <strong>Results: </strong>The results showed that expression of Lig1 gene in operating room personnel has a significant increase compared with the control group (p.0.001). After applying the 2Gy challenge dose, the expression of this gene was significantly reduced in the operating room personnel. Nonetheless, in comparison with the control group, a significant difference was observed. Regarding the changes of Ku80 and P53 genes expressions in operating room personnel, no significant difference was observed (P>0.05). However, P53 had a quite significant increase compared with the control group (p.0.001). <strong>Conclusion: </strong>The results of the study showed that the exposure to challenge dose of ionizing radiation can create compatibility and adaptive response in expression of the effective genes <span style="text-decoration: underline;"> </span> <span style="text-decoration: underline;"> for DNA repair such as Ku80 and P53 in operating room personnel.</span>