Evaluation of Cisplatin Efficacy on HepG2 and E. coli Cells under Acidic Conditions

Abstract

Background: Cisplatin (Cispt) is a common anticancer drug for the treatment of several malignancies, including<br />hepatocarcinoma. However, this drug suffers from instability in aqueous solutions. The study aimed to evaluate cisplatin<br />efficacy on HepG2 and E. coli cells under an acidic condition. Methods: Acidic Cispt was prepared via incubation in<br />acidic condition (pH=2) for a month duration. The chemical structure of the acidic Cispt was evaluated by using Fourier<br />Transform Infrared Spectroscopy (FTIR) method. The cytotoxicity of the standard and acidic Cispt were then determined<br />by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and minimum inhibitory concentration (MIC)<br />assays on HepG2 and E. coli cells, respectively. Results: After preparing of acidic Cispt, its chemical structure was<br />determined by FTIR method. In addition, cytotoxicity effects of Cispt in the standard and acidic forms were calculated<br />58 ± 2.9 and 65 ± 3.25 μM, respectively. MIC results also confirmed the results of MTT assay. MIC results for the<br />standard and acidic Cispt were estimated 9.5 ± 0.47 and 9.8 ± 0.49 μM, respectively. Conclusion: Preparing Cispt in<br />acidic condition not only did not degrade the drug, but also kept the potency of the drug approximately equal to parent<br />drug. Regarding the instability issues of Cispt, keeping Cispt in acidic condition could be a promising approach to<br />preserve its efficacy.