Evaluation the Anti-Cancer Effect of PEGylated Nano-Niosomal Gingerol, on Breast Cancer Cell lines (T47D), In-Vitro

Abstract

Background: Cancer is a significant problem in modern medicine, also is the most common cause of death after<br />cardiovascular diseases, and in need of targeted drug release. Although, chemotherapy is an important candidate in<br />cancer treatment, but it has many side effects on healthy tissues of the body. Therefore, Nano technology is used<br />for specific function, by the least side effects and damage to normal cells. Materials and method: In this study, the<br />pharmacological properties of PEGylated Nano-niosomal Gingerol was examined. Noisome were prepared using reverse<br />phase evaporation method, which contains specific proportion of cholesterol, span60 and polyethylene glycol. Then,<br />PEGylated the prepared formulation by PEG6600. The amount of release and encapsulation of the drug was investigated.<br />The percentage of remains of cancer cell line T47D treated with PEGylated niosomal Gingerol. Results: The average<br />diameter of the nanoparticles, size distribution and zeta potential were reported for PEGylated niosomal sample 35.65<br />nm, 0.17 and 21 mv, and for PEGylated niosomal drug sample 256.9 nm, 0.23 and 28 mv, respectively. The amount<br />of OD for encapsulated drug was 0.198, also the amount of concentration of the drug which is not encapsulated, was<br />0.77947 μl of the drug per ml. This value of encapsulated drug was 76.38 percent. Conclusion: The results showed that<br />IC50 of the formulation of PEGylated nanoniosomal Gingerol is less than the standard drug. It seems, the cause of this<br />phenomenon is due to the effect of Polyethylene glycol, in more stability and slower drug release, in the formulation<br />of PEGylated niosome. Also, Polyethylene glycol makes increase in the drug dealing and its greater influence with the<br />target cell. In this study, more than 76% of the Gingerol drug in PEGylated nanoniosomal formulation were enclose.<br />Also, we could reduce the amount of drug release, as much as possible.