Detection of icaABCD Genes and Biofilm Formation in Clinical Isolates of Methicillin Resistant Staphylococcus aureus

Abstract

Background & Objectives: Methicillin resistance <em>Staphylococcus aureus </em>(MRSA) is one of the most important pathogens that causes several nosocomial and community infections. Adhesion to surfaces and biofilm formation is considered main step in staphylococcal infection. The aims of this study were to determine presence of<em>icaABCD </em>genes and relation to the biofilm formation in of MRSA isolates. Methods: Of the 63 MRSA clinical isolates collected from selected hospitals in Tehran, Iran,quantitative biofilm formation was determined by microtiter tissue culture plates (MtP). All MRSA isolates were examined for determination the <em>icaABCD </em>genes by using PCR method. Results: twenty nine (46%) of the isolates were strong produced biofilm on Mtp. All of the MRSA isolates carried <em>icaD</em> and <em>icaC</em> genes, whereas, the prevalence of <em>icaA </em>and <em>icaB </em>was 60.3% and 51% respectively. Conclusions: <em>S. aureus</em> clinical isolates have different capacity to production biofilm and adhesion. This may be caused by a different in the expression of biofilm genes and hetrogenicity in genetic origins.