Evaluation of phytochemical and antioxidant activity in different parts of Heracleum gorganicum Rech.F. in Golestan province of Iran. Masoumeh Mazandarani1*, Samira Makari 2, Gholam Reza Bajian2, Parastoo Zarghami Moghaddam3 and Mehdi Abrodi4

Abstract

<span style="font-family: Calibri;"><span style="font-size: medium;"><span><span> </span>The aim of the present experiment was to study the relationship between secondary metabolites content (phenol and flavonoid) and antioxidant activity in root, stem, leaves, and fruits</span><span> of </span><em><span>Heracleum </span></em><em><span>gorganicum</span></em><span>Rech. F. The various parts of plant were collected in Ziarat Mountainin Golestan province. <span>Total phenolics (TP) and total flavonoids (TF) contents </span>were determined spectrophotometrically<span> and antioxidant activity was measured by </span>1,1-diphenyl-2-picryl hydrazyl </span><span>radical scavenging</span><span> (DPPH) method</span><span>. Results showed that the TP varied from </span><span>1.31±0.22 to 14.6±0.52 </span></span><span><span style="font-size: medium;">mg GAE g </span>^{<span style="font-size: small;">-1</span>}<span style="font-size: medium;"> in the parts and TF contents were between 2 ±0.5 to84.84 ± 11.65 mg QUE g</span>^{<span style="font-size: small;">-1</span>}<span style="font-size: medium;">. Antioxidant Activity(IC 50)</span></span><span style="font-size: medium;"><span>was measured in ranges 0.11</span><span>±0.01 to 0.23±0.015</span></span><span><span style="font-size: medium;">mg ml</span>^{<span style="font-size: small;">-1 </span>}</span><span style="font-size: medium;"><span>in DPPH method. </span><span>There was a positive correlation between antioxidant activity and secondary metabolites content. The results of the present study showed that the leaves of </span><em><span>H. </span></em><em><span>gorganicum</span></em><span> had the highest content of TP and TF compounds and antioxidant activity, providing natural sources for antioxidant compounds.<span> This confirmed traditional uses of the plant by the rural healers as </span></span><span>antiseptic, digestive, carminative and epilepsy in north of Iran</span><span>.</span></span></span> <strong><span> </span></strong>