Antimigration Activity of an Ethylacetate Fraction of Zanthoxylum acanthopodium DC. Fruits in 4T1 Breast Cancer Cells

Abstract

Objective: This study was carried out to investigate the antimigration activity of Zanthoxylum acanthopodium DC.<br />in the 4T1 breast cancer cell line. Methods: Zanthoxylum acanthopodium DC. fruit powder was extracted by maceration<br />method with n-hexane and ethylacetate solvents. Cytotoxicity and proliferation were assessed using the MTT method<br />and the cell cycle by flow cytometry. In addition, wound healing assays were conducted by a microscopic method,<br />and expression of COX-2 and VEGFR-2 were determined using qRT-PCR. Results: The IC50 of the ethylacetate<br />fraction (EAF) was 48.1 ± 1.06 μg/mL. The EAE at a concentration 10 μg/mL with viable cells was 62.3 ± 0.28%<br />after 72 h incubation, with accumulation in the G2-M phase, inhibition of cell migration in the wound healing assay,<br />and decrease in expression of COX-2 (0.62 ± 0.01) and VEGFR-2 (0.39 ± 0.003). Conclusion: The results reveal that<br />an ethylacetate fraction of Zanthoxylum acanthopodium DC. fruits provides effective antimigration effects. Further<br />studies are now planned to assess the potential of the ethylacetate fraction to inhibit angiogenesis in breast cancer and<br />determine underlying mechanisms.