The in vitro Effect of Doxorubicine-G2-FA Treatment on Breast Cancer Copyright

Abstract

BACKGROUND:<br /> <span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA"><span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA">To achieve delivery of a drug to tumors, folic acid (FA) was used as a targeting ligand to</span></span><br /> change nanocarriers. Since the folate receptor has more expression in several tumor types such as breast cancer.<br /> OBJECTIVES:<br /> <span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA"><span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA">The present study aimed to evaluate the effect of Doxorubicine-G2-FA (Dox- G2-FA) with</span></span><br /> in vitro assays. The abbreviation of G2 represents the second generation of dendrimer synthesis.<br /> METHODS:<br /> <span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA"><span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA">For this purpose, Dox-G2-FA was synthesized and mass spectroscopy was used to confirm the</span></span><br /> synthesized component. Also, MTT assay, flow cytometry, and gene expression assay by real-time PCR were<br /> used to evaluate cell viability, apoptosis, and necrosis.<br /> RESULTS:<br /> <span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA"><span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA">In this study, the effect of Dox and Dox-G2-FA on the expression of Bax, Bcl2 genes showed</span></span><br /> that there was a significant decrease in the expression of the Bcl2 gene in the Dox-G2-FA group compared to<br /> Dox and control groups(<br /> <br /> <em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: medium;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: medium;">P</span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA"><span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA"><0.05). Also, the results of flow-cytometry showed that apoptosis in the presence of</span></span><br /> Dox-G2-FA was greater than in the Dox group (<br /> <br /> <em><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: medium;"><span style="font-family: TimesNewRomanPS-ItalicMT; font-size: medium;">P</span></span></em><span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA"><span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA"><0.05).</span></span><br /> CONCLUSIONS:<br /> <span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA"><span style="font-family: TimesNewRomanPSMT; font-size: medium;" lang="JA">Therefore, it seems that the effect of Dox-G2-FA on apoptosis is better than the effect of</span></span><br /> Dox usage alone.