Effects of bisphenol A and DDT on mRNA expression of vitellogenin II in liver of quail embryos

Abstract

This study was conducted to reveal the estrogenic effects of bisphenol A and o, p'-DDT on quailembryos. Thirteen fertilized eggs were used as control (injected with 20 μl corn oil), 15 eggs were injectedwith estradiol 17β (0.04 mg dissolved in 20 μl corn oil), 20 eggs were injected with BPA (2 mg dissolved in20 μl corn oil) and 20 eggs were injected with o, p'-DDT (2 mg dissolved in 20 μl corn oil) at day 13 ofincubation. Two days later the livers of the embryos were collected. The DNA was extracted from the liverfor molecular sexing, while total RNA was extracted for vitellogenin II (VTGII) mRNA expression inembryos. In female embryos, BPA and o, p'-DDT induced variable levels of VTGII mRNA expression,while in male embryos, o, p'-DDT induced a slightly VTGII mRNA expression. In contrast, there was noexpression of VTGII after BPA injection. In conclusion, the estrogenicity of BPA was lower than o, p'-DDTand both of them were lower than the estradiol 17β.